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BIOL 359

This course will focus upon the principles of isolation, propagation, characterization and preservation of animal and plant cell lines. A variety of culture techniques will be examined and performed including media preparation, maintenance of in vitro cells lines, plasmid transfection, clone selection by limited dilution and protoplast fusion hybridization of plant based somatic cell lines.  The latter half of the course will focus on applied immunological topics and practices such as Immunoprecipitation, Western blots and Enzyme Linked ImmunoSorbant Assays (ELISA).

Prerequisite: BIOL 240             Corequisites: BIOL350